Coding

Part:BBa_K2398503:Design

Designed by: Thore Buergel, Lukas Adam, Catharina Gandor, Marita Klein, Jan Mathony, Pauline Pfuderer, Lukas Platz, Moritz Przybilla, Max Schwendemann, Julius Upmeier zu Belzen   Group: iGEM17_Heidelberg   (2017-10-25)


beta-lactamase_ Y103D_M67G_F70G This part is the coding sequence of the E. coli beta-lactamase.

In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

compatible with RFC10 and Goldengate


Source

genomic, with modifications

References